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Apoe deficiency increases neutral lipid accumulation in lipid-loaded BMDMs (A) Schematic overview of BMDM isolation and BODIPY 493/503 staining. Bone marrow cells were isolated from WT and Apoe −/− mice and differentiated into BMDMs with M-CSF for 7 days. (B–D) Representative flow cytometry histograms and quantification of BODIPY 493/503 signal in WT and Apoe −/− BMDMs under untreated conditions (B) , after OA treatment (200 μM, 24 h) (C) , and after oxLDL treatment (50 μg/mL, 24 h) (D) . BODIPY 493/503 fluorescence was quantified as background-subtracted median fluorescence intensity (ΔMedian MFI). Data are shown as mean ± SEM. n = 3 biological replicates per group. P values were calculated using unpaired two-tailed Welch’s t-tests. ns, not significant; ** P < 0.01 ; *** P < 0.001 . OA, oleic acid; oxLDL, oxidized low-density lipoprotein; M-CSF, <t>macrophage</t> <t>colony-stimulating</t> factor; BMDMs, bone marrow-derived macrophages.
Macrophage Colony, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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macrophage colony - by Bioz Stars, 2026-09
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recombinant macrophage colony  (MedChemExpress)
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Apoe deficiency increases neutral lipid accumulation in lipid-loaded BMDMs (A) Schematic overview of BMDM isolation and BODIPY 493/503 staining. Bone marrow cells were isolated from WT and Apoe −/− mice and differentiated into BMDMs with M-CSF for 7 days. (B–D) Representative flow cytometry histograms and quantification of BODIPY 493/503 signal in WT and Apoe −/− BMDMs under untreated conditions (B) , after OA treatment (200 μM, 24 h) (C) , and after oxLDL treatment (50 μg/mL, 24 h) (D) . BODIPY 493/503 fluorescence was quantified as background-subtracted median fluorescence intensity (ΔMedian MFI). Data are shown as mean ± SEM. n = 3 biological replicates per group. P values were calculated using unpaired two-tailed Welch’s t-tests. ns, not significant; ** P < 0.01 ; *** P < 0.001 . OA, oleic acid; oxLDL, oxidized low-density lipoprotein; M-CSF, <t>macrophage</t> <t>colony-stimulating</t> factor; BMDMs, bone marrow-derived macrophages.
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recombinant macrophage colony - by Bioz Stars, 2026-09
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murine recombinant m csf  (MedChemExpress)
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Apoe deficiency increases neutral lipid accumulation in lipid-loaded BMDMs (A) Schematic overview of BMDM isolation and BODIPY 493/503 staining. Bone marrow cells were isolated from WT and Apoe −/− mice and differentiated into BMDMs with M-CSF for 7 days. (B–D) Representative flow cytometry histograms and quantification of BODIPY 493/503 signal in WT and Apoe −/− BMDMs under untreated conditions (B) , after OA treatment (200 μM, 24 h) (C) , and after oxLDL treatment (50 μg/mL, 24 h) (D) . BODIPY 493/503 fluorescence was quantified as background-subtracted median fluorescence intensity (ΔMedian MFI). Data are shown as mean ± SEM. n = 3 biological replicates per group. P values were calculated using unpaired two-tailed Welch’s t-tests. ns, not significant; ** P < 0.01 ; *** P < 0.001 . OA, oleic acid; oxLDL, oxidized low-density lipoprotein; M-CSF, <t>macrophage</t> <t>colony-stimulating</t> factor; BMDMs, bone marrow-derived macrophages.
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murine recombinant m csf - by Bioz Stars, 2026-09
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mouse m csf  (MedChemExpress)
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Apoe deficiency increases neutral lipid accumulation in lipid-loaded BMDMs (A) Schematic overview of BMDM isolation and BODIPY 493/503 staining. Bone marrow cells were isolated from WT and Apoe −/− mice and differentiated into BMDMs with M-CSF for 7 days. (B–D) Representative flow cytometry histograms and quantification of BODIPY 493/503 signal in WT and Apoe −/− BMDMs under untreated conditions (B) , after OA treatment (200 μM, 24 h) (C) , and after oxLDL treatment (50 μg/mL, 24 h) (D) . BODIPY 493/503 fluorescence was quantified as background-subtracted median fluorescence intensity (ΔMedian MFI). Data are shown as mean ± SEM. n = 3 biological replicates per group. P values were calculated using unpaired two-tailed Welch’s t-tests. ns, not significant; ** P < 0.01 ; *** P < 0.001 . OA, oleic acid; oxLDL, oxidized low-density lipoprotein; M-CSF, <t>macrophage</t> <t>colony-stimulating</t> factor; BMDMs, bone marrow-derived macrophages.
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Apoe deficiency increases neutral lipid accumulation in lipid-loaded BMDMs (A) Schematic overview of BMDM isolation and BODIPY 493/503 staining. Bone marrow cells were isolated from WT and Apoe −/− mice and differentiated into BMDMs with M-CSF for 7 days. (B–D) Representative flow cytometry histograms and quantification of BODIPY 493/503 signal in WT and Apoe −/− BMDMs under untreated conditions (B) , after OA treatment (200 μM, 24 h) (C) , and after oxLDL treatment (50 μg/mL, 24 h) (D) . BODIPY 493/503 fluorescence was quantified as background-subtracted median fluorescence intensity (ΔMedian MFI). Data are shown as mean ± SEM. n = 3 biological replicates per group. P values were calculated using unpaired two-tailed Welch’s t-tests. ns, not significant; ** P < 0.01 ; *** P < 0.001 . OA, oleic acid; oxLDL, oxidized low-density lipoprotein; M-CSF, <t>macrophage</t> <t>colony-stimulating</t> factor; BMDMs, bone marrow-derived macrophages.
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mouse mce cat - by Bioz Stars, 2026-09
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m csf  (MedChemExpress)
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Apoe deficiency increases neutral lipid accumulation in lipid-loaded BMDMs (A) Schematic overview of BMDM isolation and BODIPY 493/503 staining. Bone marrow cells were isolated from WT and Apoe −/− mice and differentiated into BMDMs with M-CSF for 7 days. (B–D) Representative flow cytometry histograms and quantification of BODIPY 493/503 signal in WT and Apoe −/− BMDMs under untreated conditions (B) , after OA treatment (200 μM, 24 h) (C) , and after oxLDL treatment (50 μg/mL, 24 h) (D) . BODIPY 493/503 fluorescence was quantified as background-subtracted median fluorescence intensity (ΔMedian MFI). Data are shown as mean ± SEM. n = 3 biological replicates per group. P values were calculated using unpaired two-tailed Welch’s t-tests. ns, not significant; ** P < 0.01 ; *** P < 0.001 . OA, oleic acid; oxLDL, oxidized low-density lipoprotein; M-CSF, <t>macrophage</t> <t>colony-stimulating</t> factor; BMDMs, bone marrow-derived macrophages.
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MedChemExpress macrophage colony stimulating factor
Apoe deficiency increases neutral lipid accumulation in lipid-loaded BMDMs (A) Schematic overview of BMDM isolation and BODIPY 493/503 staining. Bone marrow cells were isolated from WT and Apoe −/− mice and differentiated into BMDMs with M-CSF for 7 days. (B–D) Representative flow cytometry histograms and quantification of BODIPY 493/503 signal in WT and Apoe −/− BMDMs under untreated conditions (B) , after OA treatment (200 μM, 24 h) (C) , and after oxLDL treatment (50 μg/mL, 24 h) (D) . BODIPY 493/503 fluorescence was quantified as background-subtracted median fluorescence intensity (ΔMedian MFI). Data are shown as mean ± SEM. n = 3 biological replicates per group. P values were calculated using unpaired two-tailed Welch’s t-tests. ns, not significant; ** P < 0.01 ; *** P < 0.001 . OA, oleic acid; oxLDL, oxidized low-density lipoprotein; M-CSF, <t>macrophage</t> <t>colony-stimulating</t> factor; BMDMs, bone marrow-derived macrophages.
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macrophage colony stimulating factor - by Bioz Stars, 2026-09
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monomeric m gfp  (OriGene)
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OriGene monomeric m gfp
Apoe deficiency increases neutral lipid accumulation in lipid-loaded BMDMs (A) Schematic overview of BMDM isolation and BODIPY 493/503 staining. Bone marrow cells were isolated from WT and Apoe −/− mice and differentiated into BMDMs with M-CSF for 7 days. (B–D) Representative flow cytometry histograms and quantification of BODIPY 493/503 signal in WT and Apoe −/− BMDMs under untreated conditions (B) , after OA treatment (200 μM, 24 h) (C) , and after oxLDL treatment (50 μg/mL, 24 h) (D) . BODIPY 493/503 fluorescence was quantified as background-subtracted median fluorescence intensity (ΔMedian MFI). Data are shown as mean ± SEM. n = 3 biological replicates per group. P values were calculated using unpaired two-tailed Welch’s t-tests. ns, not significant; ** P < 0.01 ; *** P < 0.001 . OA, oleic acid; oxLDL, oxidized low-density lipoprotein; M-CSF, <t>macrophage</t> <t>colony-stimulating</t> factor; BMDMs, bone marrow-derived macrophages.
Monomeric M Gfp, supplied by OriGene, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mouse(m)-cc1/Slc22a4+(NM_019687)+Mouse+Tagged+ORF+Clone/pm42246276-56-15-22
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Apoe deficiency increases neutral lipid accumulation in lipid-loaded BMDMs (A) Schematic overview of BMDM isolation and BODIPY 493/503 staining. Bone marrow cells were isolated from WT and Apoe −/− mice and differentiated into BMDMs with M-CSF for 7 days. (B–D) Representative flow cytometry histograms and quantification of BODIPY 493/503 signal in WT and Apoe −/− BMDMs under untreated conditions (B) , after OA treatment (200 μM, 24 h) (C) , and after oxLDL treatment (50 μg/mL, 24 h) (D) . BODIPY 493/503 fluorescence was quantified as background-subtracted median fluorescence intensity (ΔMedian MFI). Data are shown as mean ± SEM. n = 3 biological replicates per group. P values were calculated using unpaired two-tailed Welch’s t-tests. ns, not significant; ** P < 0.01 ; *** P < 0.001 . OA, oleic acid; oxLDL, oxidized low-density lipoprotein; M-CSF, macrophage colony-stimulating factor; BMDMs, bone marrow-derived macrophages.

Journal: Frontiers in Immunology

Article Title: APOE -associated lipid-handling macrophages in hepatocellular carcinoma: ligand–receptor communication and host Apoe -linked myeloid remodeling

doi: 10.3389/fimmu.2026.1902143

Figure Lengend Snippet: Apoe deficiency increases neutral lipid accumulation in lipid-loaded BMDMs (A) Schematic overview of BMDM isolation and BODIPY 493/503 staining. Bone marrow cells were isolated from WT and Apoe −/− mice and differentiated into BMDMs with M-CSF for 7 days. (B–D) Representative flow cytometry histograms and quantification of BODIPY 493/503 signal in WT and Apoe −/− BMDMs under untreated conditions (B) , after OA treatment (200 μM, 24 h) (C) , and after oxLDL treatment (50 μg/mL, 24 h) (D) . BODIPY 493/503 fluorescence was quantified as background-subtracted median fluorescence intensity (ΔMedian MFI). Data are shown as mean ± SEM. n = 3 biological replicates per group. P values were calculated using unpaired two-tailed Welch’s t-tests. ns, not significant; ** P < 0.01 ; *** P < 0.001 . OA, oleic acid; oxLDL, oxidized low-density lipoprotein; M-CSF, macrophage colony-stimulating factor; BMDMs, bone marrow-derived macrophages.

Article Snippet: Bone marrow cells from WT and Apoe −/− mice were differentiated into bone marrow-derived macrophages (BMDMs) with macrophage colony-stimulating factor (M-CSF; 25 ng/mL; MedChemExpress, HY-P7085) for 7 days.

Techniques: Isolation, Staining, Flow Cytometry, Fluorescence, Two Tailed Test, Derivative Assay